mouse and human spermatogonial stem cells
نویسندگان
چکیده
spermatogonial stem cells (sscs) are in the beginning of a complex process in which they transmit genetic information from generation to generation. any failure in this process can result in infertility. it has been suggested that transplantation of spermatogonial stem cells, following their maintenance and culturing, may restore fertility in some infertile patients. because fertility restoration through sscs transplantation has been successfully achieved in animal experiments, we hope human studies can follow in the near future. the isolation and cultivation of sscs help us study their biological characteristics and their application in therapeutic approaches. in this review, we studied spermatogenesis in rodents and humans. we also compared markers and different ssc culture systems in both.
منابع مشابه
Mouse and Human Spermatogonial Stem Cells
Spermatogonial stem cells (SSCs) are in the beginning of a complex process in which they transmit genetic information from generation to generation. Any failure in this process can result in infertility. It has been suggested that transplantation of spermatogonial stem cells, following their maintenance and culturing, may restore fertility in some infertile patients. Because fertility restorati...
متن کاملPhenotypic Plasticity of Mouse Spermatogonial Stem Cells
BACKGROUND Spermatogonial stem cells (SSCs) continuously undergo self-renewal division to support spermatogenesis. SSCs are thought to have a fixed phenotype, and development of a germ cell transplantation technique facilitated their characterization and prospective isolation in a deterministic manner; however, our in vitro SSC culture experiments indicated heterogeneity of cultured cells and s...
متن کاملComparison of Neonatal and Adult Mice-derived Sertoli Cells in Support of Expansion of Mouse Spermatogonial Stem Cells In vitro
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O-3: Identification and Characterization of Repopulating Spermatogonial Stem Cells from The Adult Human Testis
Background: This study was conducted to identify and characterize repopulating spermatogonial stem cells (SSCs) in the adult human testes. Materials and Methods: Testes biopsies from obstructive azoospermic patients and normal segments of human testicular tissue were used. Flow cytometry, real time PCR and immunohistochemical analysis were performed. Purified human spermatogonia were transplant...
متن کاملThe Effects of Plla Nanofiber Scaffold on Proliferation of Frozen-Thawed Neonate Mouse Spermatogonial Stem Cells
Purpose: To investigate of the effects of a poly L-lactic acid (PLLA) nanofiber scaffold on proliferation of frozen-thawed neonate mouse spermatogonial stem cells.Materials and Methods: Spermatogonial cells were isolated from neonatal 3-6-day-old NMRI mice testes by two steps enzymatic digestion and differential plating. The isolated spermatogonial cells were divided into four culture groups: 1...
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عنوان ژورنال:
cell journalجلد ۱۲، شماره ۲، صفحات ۱۴۷-۱۵۸
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